KIF23 Antibody - #DF2573
(3)
(6)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2573, RRID:AB_2839779.
折りたたみ／展開
CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1;
免疫原
- Q02241 KIF23_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSARAKTPRKPTVKKGSQTNLKDPVGVYCRVRPLGFPDQECCIEVINNTTVQLHTPEGYRLNRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSPGEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMPNPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPIKPKPPQSKLLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQMYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVDKAICGLTPGRRYRNQPRGPVGNEPLVTDVVLQSFPPLPSCEILDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSVSPSPVPLSSNYIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKIPTYNTPLKVTSIARRRQQEPGQSKTCIVSDRRRGMYWTEGREVVPTFRNEIEIEEDHCGRLLFQPDQNAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02241 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T8 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K188 | Acetylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| T450 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| T580 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| K586 | Sumoylation | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K647 | Sumoylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| K662 | Sumoylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Q9NRM7 (LATS2) , O95835 (LATS1) , Q15208 (STK38) | Uniprot |
| S718 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| T735 | Phosphorylation | Uniprot | |
| Y736 | Phosphorylation | Uniprot | |
| T738 | Phosphorylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| K757 | Ubiquitination | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| S814 | Phosphorylation | Q9NRM7 (LATS2) , O95835 (LATS1) , Q15208 (STK38) | Uniprot |
| T861 | Phosphorylation | Uniprot | |
| S867 | Phosphorylation | Uniprot | |
| T873 | Phosphorylation | Uniprot | |
| K877 | Sumoylation | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| Y881 | Phosphorylation | Uniprot | |
| T883 | Phosphorylation | Uniprot | |
| R884 | Methylation | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| T897 | Phosphorylation | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| S904 | Phosphorylation | Uniprot | |
| S905 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S912 | Phosphorylation | Uniprot | |
| T913 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| T927 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| Y949 | Phosphorylation | Uniprot | |
| K956 | Ubiquitination | Uniprot |
研究背景
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Ubiquitinated. Deubiquitinated by USP8/UBPY.
Nucleus. Cytoplasm>Cytoskeleton>Spindle. Midbody>Midbody ring.
Note: Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
参考文献
Application: IHC Species: Human Sample:
Application: WB Species: human Sample: liver cancer cell
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