SMARCAD1 Antibody - #DF2598
| 製品: | SMARCAD1 Antibody |
| カタログ: | DF2598 |
| タンパク質の説明: | Rabbit polyclonal antibody to SMARCAD1 |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 117 kDa; 117kD(Calculated). |
| ユニプロット: | Q9H4L7 |
| RRID: | AB_2839804 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2598, RRID:AB_2839804.
折りたたみ／展開
ATP dependent helicase 1; ATP-dependent helicase 1; ETL1; hHEL1; Smarcad1; SMRCD_HUMAN; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1;
免疫原
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level) (PubMed:29409814).
- Q9H4L7 SMRCD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDITEKTEDSSVPETPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTVIIVSEPSEDEESQGLPTMARRNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKLIESTSTMDGAIAAALLMFGDAGGGPRKRKLSSSSEPYEEDEFNDDQSIKKTRLDHGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFNPKRVVEDSEYDSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRPFNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSILNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNWLREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAHAKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRKMANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHINNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H4L7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K77 | Sumoylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K84 | Sumoylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K161 | Sumoylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K258 | Sumoylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K268 | Methylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| K335 | Sumoylation | Uniprot | |
| K346 | Sumoylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K695 | Sumoylation | Uniprot | |
| K695 | Ubiquitination | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| K716 | Sumoylation | Uniprot | |
| K724 | Sumoylation | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| K729 | Acetylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| K767 | Ubiquitination | Uniprot | |
| K780 | Ubiquitination | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| T880 | Phosphorylation | Uniprot | |
| Y897 | Phosphorylation | Uniprot | |
| K903 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| K994 | Acetylation | Uniprot |
研究背景
DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.
Nucleus. Chromosome.
Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage.
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).
Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA.
Belongs to the SNF2/RAD54 helicase family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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