ZDHHC13 Antibody - #DF2600

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製品: ZDHHC13 Antibody
カタログ: DF2600
タンパク質の説明: Rabbit polyclonal antibody to ZDHHC13
アプリケーション: WB IHC
反応性: Human, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 58 kDa; 71kD(Calculated).
ユニプロット: Q8IUH4
RRID: AB_2839806

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Monkey
予測:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
ZDHHC13 Antibody detects endogenous levels of total ZDHHC13.
RRID:
AB_2839806
引用形式: Affinity Biosciences Cat# DF2600, RRID:AB_2839806.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DHHC 13; DHHC-13; FLJ10852; FLJ10941; HIP14 like; HIP14 related protein; HIP14-related protein; HIP14L; HIP3RP; Huntingtin interacting protein 14 related protein; Huntingtin interacting protein HIP3RP; Huntingtin-interacting protein 14-related protein; Huntingtin-interacting protein HIP3RP; Hypothetical protein similar to ankyrin repeat containing priotein AKR1; MGC64994; Palmitoyltransferase ZDHHC13; Probable palmitoyltransferase ZDHHC13; Putative MAPK activating protein PM03; Putative MAPK-activating protein PM03; Putative NF kappa B activating protein 209; Putative NF-kappa-B-activating protein 209; ZDH13_HUMAN; ZDHHC 13; ZDHHC13; Zinc finger DHHC domain containing 13; Zinc finger DHHC domain-containing protein 13;

免疫原

免疫原:
Uniprot:

Q8IUH4(ZDH13_HUMAN) >>Visit HPA database.

遺伝子(ID):
ZDHHC13(54503)
タンパク質の説明:
Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport.
タンパク質配列:
MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLIEDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDKENVSLLHWAAINNRLDLVKFYISKGAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMPIIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNPSLNVVDKIHQNTPLHWAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMRANQKFRLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVGYKNLVYLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDPGFTKASEEEKKVNIITLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLWTGRCIGFGNHHYYIFFLFFLSMVCGWIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACSPWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IUH4 基板として

Site PTM Type Enzyme
M1 Acetylation
S8 Phosphorylation
Y25 Phosphorylation
K33 Ubiquitination
K55 Ubiquitination
Y59 Phosphorylation
K66 Ubiquitination
Y73 Phosphorylation
R76 Methylation
K80 Ubiquitination
K192 Ubiquitination
K214 Ubiquitination
K235 Ubiquitination
K249 Ubiquitination
K261 Ubiquitination
K566 Ubiquitination

研究背景

機能:

Palmitoyltransferase for HTT and GAD2. May play a role in Mg(2+) transport.

細胞の位置付け:

Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane>Multi-pass membrane protein.
Note: Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts (via ANK repeats) with CLIP3. Interacts (via ANK repeats) with DNAJC5 (via C-terminus) (By similarity). Interacts (via ANK repeats) with HTT. Interacts (via ANK repeats) with MAP6. Interacts (via ANK repeats) with SNAP23. Interacts (via ANK repeats) with SNAP25. May interact (via ANK repeats) with SPRED2 (By similarity).

タンパク質ファミリー:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.

参考文献

1). A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chemical Biology, 2021 (PubMed: 34458782) [IF=4.1]

Application: WB    Species: Human    Sample: A375 cells

Fig. 3 PD-1 is palmitoylated by DHHC9. (A) Western blot showing the effect of two independent siRNAs targeting the indicated DHHC enzymes on DHHC protein levels in A375 cells with the specific antibody for each DHHC enzyme. (B) Western blot showing the expression of PD-1 in A375 cells treated by two independent siRNAs targeting the indicated DHHC enzymes. (C) Western blot showing the expression of PD-1 in RKO and HCT116 cells treated by two independent siRNAs targeting DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments. (D) Immunoblot showing the expression of PD-1 in different cells overexpressing DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments.

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