SOX4 Antibody - #DF2610
(5)
(3)
| 製品: | SOX4 Antibody |
| カタログ: | DF2610 |
| タンパク質の説明: | Rabbit polyclonal antibody to SOX4 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Rabbit, Dog |
| 分子量: | 47 kDa; 47kD(Calculated). |
| ユニプロット: | Q06945 |
| RRID: | AB_2839816 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Rabbit(86%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SOX4 Antibody detects endogenous levels of total SOX4.
RRID:
AB_2839816
引用形式: Affinity Biosciences Cat# DF2610, RRID:AB_2839816.
引用形式: Affinity Biosciences Cat# DF2610, RRID:AB_2839816.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
Ecotropic viral integration site 16; EVI 16; EVI16; Sox4; SOX4_HUMAN; SRY (sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; Transcription factor SOX-4;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質の説明:
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
タンパク質配列:
- Q06945 SOX4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSSSSAAASSKPGEKGDKVGGSGGGGHGGGGGGGSSNAGGGGGGASGGGANSKPAQKKSCGSKVAGGAGGGVSKPHAKLILAGGGGGGKAAAAAAASFAAEQAGAAALLPLGAAADHHSLYKARTPSASASASSAASASAALAAPGKHLAEKKVKRVYLFGGLGTSSSPVGGVGAGADPSDPLGLYEEEGAGCSPDAPSLSGRSSAASSPAAGRSPADHRGYASLRAASPAPSSAPSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYCTPEVSEMISGDWLESSISNLVFTY
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
86
Chicken
78
Xenopus
67
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q06945 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S50 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| R75 | Methylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K213 | Acetylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot |
研究背景
機能:
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
細胞の位置付け:
Nucleus.
組織特異性:
Testis, brain, and heart.
サブユニット構造:
Interacts with UBE2I.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
参考文献
1). SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. CELL DEATH AND DIFFERENTIATION, 2024
[IF=13.7]
2). Schizandrin A can inhibit non‑small cell lung cancer cell proliferation by inducing cell cycle arrest, apoptosis and autophagy. International Journal of Molecular Medicine, 2021
(PubMed: 34643254)
[IF=5.4]
Application: WB Species: Human Sample: A549 and H1975 cells
Figure 2 SchA induces cell cycle arrest in A549 and H1975 cells. The effect of SchA on the cell cycle progression of (A) A549 and (B) H1975 cells. The effect of SchA on the protein expression levels of cell cycle regulation proteins in (C) A549 and (D) H1975 cells. *P
3). Expression of SOX4 Significantly Predicts the Risk of Lymph Node Metastasis for Patients With Early-Stage Esophageal Squamous Cell Carcinoma. Laboratory investigation; a journal of technical methods and pathology, 2024
(PubMed: 38431117)
[IF=5.0]
4). Circ_0089823 reinforces malignant behaviors of non-small cell lung cancer by acting as a sponge for microRNAs targeting SOX4. NEOPLASIA, 2021
(PubMed: 34311177)
[IF=4.8]
Application: WB Species: Human Sample: NSCLC cells
Fig. 6. SOX4 is targeted by miR-507, miR-557, miR-579-3p and miR-1287-5p. (A-D) The binding between SOX4 and these 4 microRNAs was verified by
luciferase reporter assay. (E-H) After transfection with mimics or inhibitor of these 4 microRNAs, the level of SOX4 was detected by qPCR. (I-L) The level of
SOX4 in NSCLC cells was determined by western blot after transfection. The results were presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
5). METTL3-mediated ALDH m6A methylation regulates the malignant behavior of BMI1+ HNSCC stem cells. Oral Diseases, 2023
(PubMed: 37249063)
[IF=3.8]
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