製品: PRKG1 Antibody
カタログ: DF2677
タンパク質の説明: Rabbit polyclonal antibody to PRKG1
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 76 kDa; 76kD(Calculated).
ユニプロット: Q13976
RRID: AB_2839883

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
PRKG1 Antibody detects endogenous levels of total PRKG1.
RRID:
AB_2839883
引用形式: Affinity Biosciences Cat# DF2677, RRID:AB_2839883.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

cGK 1; cGK1; cGKI alpha; cGKI; cGKI beta; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1_HUMAN; KGPB; MGC71944; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13976 KGP1_HUMAN:

Primarily expressed in lung and placenta.

タンパク質配列:
MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
92
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13976 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S27 Phosphorylation
S51 Phosphorylation
T59 Phosphorylation P17252 (PRKCA)
S65 Phosphorylation Q13976 (PRKG1)
T85 Phosphorylation
Y212 Phosphorylation
S218 Phosphorylation
S231 Phosphorylation
Y247 Phosphorylation
S273 Phosphorylation
Y433 Phosphorylation
Y435 Phosphorylation
Y497 Phosphorylation
T515 Phosphorylation
T517 Phosphorylation
K607 Acetylation
K615 Acetylation
K617 Acetylation

PTMs - Q13976 酵素として

Substrate Site Source
O14974 (PPP1R12A) S692 Uniprot
O14974 (PPP1R12A) S695 Uniprot
O14974 (PPP1R12A) S852 Uniprot
O76074-2 (PDE5A) S60 Uniprot
O76074-1 (PDE5A) S102 Uniprot
O96001-1 (PPP1R17) T68 Uniprot
O96001-1 (PPP1R17) T119 Uniprot
P04792 (HSPB1) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P04792 (HSPB1) T143 Uniprot
P10451-5 (SPP1) S148 Uniprot
P11831 (SRF) T159 Uniprot
P13569 (CFTR) S660 Uniprot
P13569-1 (CFTR) S700 Uniprot
P13569-1 (CFTR) S737 Uniprot
P13569-1 (CFTR) S768 Uniprot
P13569 (CFTR) S795 Uniprot
P13569-1 (CFTR) S813 Uniprot
P16220-1 (CREB1) S133 Uniprot
P19429 (TNNI3) S23 Uniprot
P19429 (TNNI3) S24 Uniprot
P21731-2 (TBXA2R) S330 Uniprot
P21731-2 (TBXA2R) T332 Uniprot
P21817-1 (RYR1) S2843 Uniprot
P24046-1 (GABRR1) S444 Uniprot
P31645 (SLC6A4) T276 Uniprot
P35367 (HRH1) S396 Uniprot
P35367 (HRH1) S398 Uniprot
P35520 (CBS) S227 Uniprot
P35520 (CBS) S525 Uniprot
P41220-1 (RGS2) S46 Uniprot
P41220 (RGS2) S64 Uniprot
P50552 (VASP) S157 Uniprot
P50552 (VASP) S239 Uniprot
P50552 (VASP) T278 Uniprot
P52943-1 (CRIP2) S104 Uniprot
P61224 (RAP1B) S179 Uniprot
P61586 (RHOA) S188 Uniprot
P78347-2 (GTF2I) S371 Uniprot
P78347-4 (GTF2I) S391 Uniprot
P78347-3 (GTF2I) S392 Uniprot
P78347 (GTF2I) S412 Uniprot
P78347-2 (GTF2I) S743 Uniprot
P78347-4 (GTF2I) S763 Uniprot
P78347-3 (GTF2I) S764 Uniprot
P78347 (GTF2I) S784 Uniprot
Q01970-1 (PLCB3) S26 Uniprot
Q01970-1 (PLCB3) S1105 Uniprot
Q03393 (PTS) S19 Uniprot
Q05469 (LIPE) S853 Uniprot
Q12791 (KCNMA1) S811 Uniprot
Q12791 (KCNMA1) S992 Uniprot
Q12791 (KCNMA1) S1204 Uniprot
Q13153 (PAK1) S21 Uniprot
Q13507-3 (TRPC3) T11 Uniprot
Q13507 (TRPC3) S251 Uniprot
Q13507-3 (TRPC3) S263 Uniprot
Q13976 (PRKG1) S65 Uniprot
Q14847 (LASP1) S146 Uniprot
Q15052 (ARHGEF6) S684 Uniprot
Q15637-1 (SF1) S20 Uniprot
Q5VSY0-1 (GKAP1) S106 Uniprot
Q684P5 (RAP1GAP2) S7 Uniprot
Q76M96 (CCDC80) S409 Uniprot
Q76M96 (CCDC80) S434 Uniprot
Q8WZ42 (TTN) S4185 Uniprot
Q92934 (BAD) S118 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96D31 (ORAI1) S34 Uniprot
Q9HCX4 (TRPC7) T15 Uniprot
Q9NS28 (RGS18) S216 Uniprot
Q9UH03-1 (SEPT3) S91 Uniprot
Q9UIK4 (DAPK2) S299 Uniprot
Q9UIK4 (DAPK2) S367 Uniprot
Q9UIK4 (DAPK2) S368 Uniprot
Q9Y210 (TRPC6) T70 Uniprot
Q9Y210 (TRPC6) S322 Uniprot
Q9Y613 (FHOD1) S1131 Uniprot
Q9Y6F6 (MRVI1) S657 Uniprot

研究背景

機能:

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

PTMs:

Autophosphorylation increases kinase activity.

65 kDa monomer is produced by proteolytic cleavage.

細胞の位置付け:

Cytoplasm.
Note: Colocalized with TRPC7 in the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Primarily expressed in lung and placenta.

サブユニット構造:

Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A. This interaction targets PRKG1 to stress fibers to mediate smooth muscle cell relaxation and vasodilation in responses to rises in cGMP. Isoform beta: antiparallel homodimer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 (By similarity). Interacts with MRVI1. Forms a stable complex with ITPR1, MRVI1, and isoform beta of PRKG1. Interacts with TRPC7 (via ankyrin repeat domain). Isoform alpha interacts with RGS2. Interacts with GTF2I.

タンパク質ファミリー:

Composed of an N-terminal leucine-zipper domain followed by an autoinhibitory domain, which mediate homodimer formation and inhibit kinase activity, respectively. Next, two cGMP-binding domains are followed by the catalytic domain at the C-terminus. Binding of cGMP to cGMP-binding domains results in a conformational change that activates kinase activity by removing the autoinhibitory domain from the catalytic cleft leaving the catalytic domain free to phosphorylate downstream substrates. Isoforms alpha and beta have identical cGMP-binding and catalytic domains but differ in their leucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Digestive system > Salivary secretion.

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