PRKG1 Antibody - #DF2677
製品: | PRKG1 Antibody |
カタログ: | DF2677 |
タンパク質の説明: | Rabbit polyclonal antibody to PRKG1 |
アプリケーション: | WB |
反応性: | Human, Mouse |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 76 kDa; 76kD(Calculated). |
ユニプロット: | Q13976 |
RRID: | AB_2839883 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2677, RRID:AB_2839883.
折りたたみ/展開
cGK 1; cGK1; cGKI alpha; cGKI; cGKI beta; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1_HUMAN; KGPB; MGC71944; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B;
免疫原
- Q13976 KGP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13976 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
S27 | Phosphorylation | Uniprot | |
S51 | Phosphorylation | Uniprot | |
T59 | Phosphorylation | P17252 (PRKCA) | Uniprot |
S65 | Phosphorylation | Q13976 (PRKG1) | Uniprot |
T85 | Phosphorylation | Uniprot | |
Y212 | Phosphorylation | Uniprot | |
S218 | Phosphorylation | Uniprot | |
S231 | Phosphorylation | Uniprot | |
Y247 | Phosphorylation | Uniprot | |
S273 | Phosphorylation | Uniprot | |
Y433 | Phosphorylation | Uniprot | |
Y435 | Phosphorylation | Uniprot | |
Y497 | Phosphorylation | Uniprot | |
T515 | Phosphorylation | Uniprot | |
T517 | Phosphorylation | Uniprot | |
K607 | Acetylation | Uniprot | |
K615 | Acetylation | Uniprot | |
K617 | Acetylation | Uniprot |
PTMs - Q13976 酵素として
Substrate | Site | Source |
---|---|---|
O14974 (PPP1R12A) | S692 | Uniprot |
O14974 (PPP1R12A) | S695 | Uniprot |
O14974 (PPP1R12A) | S852 | Uniprot |
O76074-2 (PDE5A) | S60 | Uniprot |
O76074-1 (PDE5A) | S102 | Uniprot |
O96001-1 (PPP1R17) | T68 | Uniprot |
O96001-1 (PPP1R17) | T119 | Uniprot |
P04792 (HSPB1) | S15 | Uniprot |
P04792 (HSPB1) | S78 | Uniprot |
P04792 (HSPB1) | S82 | Uniprot |
P04792 (HSPB1) | T143 | Uniprot |
P10451-5 (SPP1) | S148 | Uniprot |
P11831 (SRF) | T159 | Uniprot |
P13569 (CFTR) | S660 | Uniprot |
P13569-1 (CFTR) | S700 | Uniprot |
P13569-1 (CFTR) | S737 | Uniprot |
P13569-1 (CFTR) | S768 | Uniprot |
P13569 (CFTR) | S795 | Uniprot |
P13569-1 (CFTR) | S813 | Uniprot |
P16220-1 (CREB1) | S133 | Uniprot |
P19429 (TNNI3) | S23 | Uniprot |
P19429 (TNNI3) | S24 | Uniprot |
P21731-2 (TBXA2R) | S330 | Uniprot |
P21731-2 (TBXA2R) | T332 | Uniprot |
P21817-1 (RYR1) | S2843 | Uniprot |
P24046-1 (GABRR1) | S444 | Uniprot |
P31645 (SLC6A4) | T276 | Uniprot |
P35367 (HRH1) | S396 | Uniprot |
P35367 (HRH1) | S398 | Uniprot |
P35520 (CBS) | S227 | Uniprot |
P35520 (CBS) | S525 | Uniprot |
P41220-1 (RGS2) | S46 | Uniprot |
P41220 (RGS2) | S64 | Uniprot |
P50552 (VASP) | S157 | Uniprot |
P50552 (VASP) | S239 | Uniprot |
P50552 (VASP) | T278 | Uniprot |
P52943-1 (CRIP2) | S104 | Uniprot |
P61224 (RAP1B) | S179 | Uniprot |
P61586 (RHOA) | S188 | Uniprot |
P78347-2 (GTF2I) | S371 | Uniprot |
P78347-4 (GTF2I) | S391 | Uniprot |
P78347-3 (GTF2I) | S392 | Uniprot |
P78347 (GTF2I) | S412 | Uniprot |
P78347-2 (GTF2I) | S743 | Uniprot |
P78347-4 (GTF2I) | S763 | Uniprot |
P78347-3 (GTF2I) | S764 | Uniprot |
P78347 (GTF2I) | S784 | Uniprot |
Q01970-1 (PLCB3) | S26 | Uniprot |
Q01970-1 (PLCB3) | S1105 | Uniprot |
Q03393 (PTS) | S19 | Uniprot |
Q05469 (LIPE) | S853 | Uniprot |
Q12791 (KCNMA1) | S811 | Uniprot |
Q12791 (KCNMA1) | S992 | Uniprot |
Q12791 (KCNMA1) | S1204 | Uniprot |
Q13153 (PAK1) | S21 | Uniprot |
Q13507-3 (TRPC3) | T11 | Uniprot |
Q13507 (TRPC3) | S251 | Uniprot |
Q13507-3 (TRPC3) | S263 | Uniprot |
Q13976 (PRKG1) | S65 | Uniprot |
Q14847 (LASP1) | S146 | Uniprot |
Q15052 (ARHGEF6) | S684 | Uniprot |
Q15637-1 (SF1) | S20 | Uniprot |
Q5VSY0-1 (GKAP1) | S106 | Uniprot |
Q684P5 (RAP1GAP2) | S7 | Uniprot |
Q76M96 (CCDC80) | S409 | Uniprot |
Q76M96 (CCDC80) | S434 | Uniprot |
Q8WZ42 (TTN) | S4185 | Uniprot |
Q92934 (BAD) | S118 | Uniprot |
Q96A00 (PPP1R14A) | T38 | Uniprot |
Q96D31 (ORAI1) | S34 | Uniprot |
Q9HCX4 (TRPC7) | T15 | Uniprot |
Q9NS28 (RGS18) | S216 | Uniprot |
Q9UH03-1 (SEPT3) | S91 | Uniprot |
Q9UIK4 (DAPK2) | S299 | Uniprot |
Q9UIK4 (DAPK2) | S367 | Uniprot |
Q9UIK4 (DAPK2) | S368 | Uniprot |
Q9Y210 (TRPC6) | T70 | Uniprot |
Q9Y210 (TRPC6) | S322 | Uniprot |
Q9Y613 (FHOD1) | S1131 | Uniprot |
Q9Y6F6 (MRVI1) | S657 | Uniprot |
研究背景
Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
Autophosphorylation increases kinase activity.
65 kDa monomer is produced by proteolytic cleavage.
Cytoplasm.
Note: Colocalized with TRPC7 in the plasma membrane.
Primarily expressed in lung and placenta.
Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A. This interaction targets PRKG1 to stress fibers to mediate smooth muscle cell relaxation and vasodilation in responses to rises in cGMP. Isoform beta: antiparallel homodimer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 (By similarity). Interacts with MRVI1. Forms a stable complex with ITPR1, MRVI1, and isoform beta of PRKG1. Interacts with TRPC7 (via ankyrin repeat domain). Isoform alpha interacts with RGS2. Interacts with GTF2I.
Composed of an N-terminal leucine-zipper domain followed by an autoinhibitory domain, which mediate homodimer formation and inhibit kinase activity, respectively. Next, two cGMP-binding domains are followed by the catalytic domain at the C-terminus. Binding of cGMP to cGMP-binding domains results in a conformational change that activates kinase activity by removing the autoinhibitory domain from the catalytic cleft leaving the catalytic domain free to phosphorylate downstream substrates. Isoforms alpha and beta have identical cGMP-binding and catalytic domains but differ in their leucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Sensory system > Olfactory transduction.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Digestive system > Salivary secretion.
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