Nkx2.5 Antibody | Affinity Biosciences

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製品:	Nkx2.5 Antibody
カタログ:	DF2699
タンパク質の説明:	Rabbit polyclonal antibody to Nkx2.5
アプリケーション:	WB IF/ICC
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Dog
分子量:	50 kDa; 35kD(Calculated).
ユニプロット:	P52952
RRID:	AB_2839905

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Dog(82%)

クローナリティ:

Polyclonal

特異性:

Nkx2.5 Antibody detects endogenous levels of total Nkx2.5.

RRID:

AB_2839905
引用形式: Affinity Biosciences Cat# DF2699, RRID:AB_2839905.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

NKX2.5, mouse, homolog of; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; HLHS2; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Homeobox protein Nkx 2.5; Homeobox protein Nkx-2.5; NK2 homeobox 5; NK2 transcription factor related locus 5; NK2 transcription factor related, locus 5 (Drosophila); NK2, Drosophila, homolog of, E; NKX2-5; NKX2.5; NKX25_HUMAN; Nkx2E; NKX4-1; Tinman paralog; VSD3;

免疫原

免疫原:

Uniprot:

P52952(NKX25_HUMAN) >>Visit HPA database.

遺伝子(ID):

NKX2-5(1482)

発現特異性:

P52952 NKX25_HUMAN:

Expressed only in the heart.

タンパク質の説明:

Receptor for VEGFC. Has a tyrosine-protein kinase activity.;

タンパク質配列:

P52952 NKX25_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Horse

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52952 基板として

P52952

Site	PTM Type	Source
S78	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot
T180	Phosphorylation	Uniprot
R225	Methylation	Uniprot

研究背景

P52952_NKX25_HUMAN

機能:

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). Binds to the core DNA motif of NPPA promoter. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.

細胞の位置付け:

Nucleus.

組織特異性:

Expressed only in the heart.

サブユニット構造:

Homodimer (via the homeobox); binds DNA as homodimer. Interacts (via the homeobox) with TBX5 (via the T-box); this complex binds DNA. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Interacts with TBX18 (By similarity).

タンパク質ファミリー:

The homeobox domain binds to double-stranded DNA (PubMed:22849347).

Belongs to the NK-2 homeobox family.

参考文献

1). Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro. PLoS One, 2015 (PubMed: 25898012) [IF=3.7]

Application: WB Species: Sample: miR-20b cells

Fig 7. |Effects of miR-20b on the BMP signaling pathway, as assessed by by western blotting. a): in the miR-20b overexpression group; b): in miR-20b silenced group. (n = 3, *: P<0.05, **: P<0.01, ***: P<0.001)

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For Research Use Only.
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Nkx2.5 Antibody - #DF2699

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MS Report

HPLC Report

MSDS

説明書

COA