5HT1D Receptor Antibody - #DF2706

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製品: 5HT1D Receptor Antibody
カタログ: DF2706
タンパク質の説明: Rabbit polyclonal antibody to 5HT1D Receptor
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
分子量: 45 kDa; 42kD(Calculated).
ユニプロット: P28221
RRID: AB_2839912

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
5HT1D Receptor Antibody detects endogenous levels of total 5HT1D Receptor.
RRID:
AB_2839912
引用形式: Affinity Biosciences Cat# DF2706, RRID:AB_2839912.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

5 HT 1D alpha; 5 HT 1D; 5 HT1B; 5 HT1D; 5 hydroxytryptamine (serotonin) receptor 1B; 5 hydroxytryptamine (serotonin) receptor 1D G protein coupled; 5 hydroxytryptamine 1B receptor (5 HT 1B) (Serotonin receptor) (5 HT 1D beta) (Serotonin 1D beta receptor) (S12); 5 hydroxytryptamine receptor 1D; HT1DA; HTR1D; HTR1D2; HTR1DA; HTRL; RDC4; S12; Serotonin receptor 1D;

免疫原

免疫原:
Uniprot:

P28221(5HT1D_HUMAN) >>Visit HPA database.

遺伝子(ID):
HTR1D(3352)
発現特異性:
P28221 5HT1D_HUMAN:

Detected in brain neocortex and caudate nucleus (at protein level).

タンパク質配列:
MSPLNQSAEGLPQEASNRSLNATETSEAWDPRTLQALKISLAVVLSVITLATVLSNAFVLTTILLTRKLHTPANYLIGSLATTDLLVSILVMPISIAYTITHTWNFGQILCDIWLSSDITCCTASILHLCVIALDRYWAITDALEYSKRRTAGHAATMIAIVWAISICISIPPLFWRQAKAQEEMSDCLVNTSQISYTIYSTCGAFYIPSVLLIILYGRIYRAARNRILNPPSLYGKRFTTAHLITGSAGSSLCSLNSSLHEGHSHSAGSPLFFNHVKIKLADSALERKRISAARERKATKILGIILGAFIICWLPFFVVSLVLPICRDSCWIHPALFDFFTWLGYLNSLINPIIYTVFNEEFRQAFQKIVPFRKAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
89
Xenopus
75
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28221 基板として

Site PTM Type Enzyme
T61 Phosphorylation
Y137 Phosphorylation
S147 Phosphorylation
S193 Phosphorylation
Y235 Phosphorylation
K280 Ubiquitination

研究背景

機能:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in brain neocortex and caudate nucleus (at protein level).

サブユニット構造:

Homodimer. Heterodimer with HTR1B.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

参考文献

1). Knockdown and inhibition of hydroxytryptamine receptor 1D suppress proliferation and migration of gastric cancer cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022 (PubMed: 35785570) [IF=3.1]

Application: IHC    Species: Mouse    Sample: subcutaneous tumors

Fig. 2. Effect of 5-hydroxytryptamine receptor 1D (HTR1D) knockdown in gastric cancer (GC) cells on HTR1D function in vitro and in vivo. Effect of HTR1D knockdown on (A) proliferation, (B) clonogenic ability, and (C) migration of AGS and MGC803 cells. Magnification of migration experiment was 200 × . (D) Subcutaneous tumor nodules were generated by subcutaneously injecting short hairpin NC (shNC) and shHTR1D-1 MGC803 cells into nude mice. (E) Tumor growth curves in shNC and shHTR1D-1 groups. (F) Weight of harvested subcutaneous tumors in shNC and shHTR1D-1 groups. (G) Immunohistochemical staining of HTR1D and Ki67 in subcutaneous tumors in shNC and shHTR1D-1 groups.

Application: WB    Species: Mouse    Sample:

figs3.

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