CCR1 Antibody - #DF2710

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製品: CCR1 Antibody
カタログ: DF2710
タンパク質の説明: Rabbit polyclonal antibody to CCR1
アプリケーション: WB IHC
反応性: Human
予測: Bovine, Horse, Sheep
分子量: 37.6 kDa; 41kD(Calculated).
ユニプロット: P32246
RRID: AB_2839916

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(80%), Horse(90%), Sheep(80%)
クローナリティ:
Polyclonal
特異性:
CCR1 Antibody detects endogenous levels of total CCR1.
RRID:
AB_2839916
引用形式: Affinity Biosciences Cat# DF2710, RRID:AB_2839916.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C C chemokine receptor type 1; C C CKR 1; C-C chemokine receptor type 1; C-C CKR-1; CC CKR 1; CC-CKR-1; CCR 1; CCR-1; CCR1; CCR1_HUMAN; CD 191; CD191; CD191 antigen; Chemokine (C C motif) receptor 1; Chemokine C C motif receptor 1; CKR 1; CKR1; CMKBR 1; CMKBR1; CMKR 1; CMKR1; HM145; LD78 receptor; Macrophage inflammatory protein 1 alpha /Rantes receptor; Macrophage inflammatory protein 1 alpha receptor; Macrophage inflammatory protein 1-alpha receptor; Mip 1a R; MIP 1alpha R; MIP-1alpha-R; MIP1aR; RANTES R; RANTES receptor; RANTES-R; SCYAR1;

免疫原

免疫原:
Uniprot:

P32246(CCR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCR1(1230)
発現特異性:
P32246 CCR1_HUMAN:

Widely expressed in different hematopoietic cells.

タンパク質の説明:
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.;
タンパク質配列:
METPNTTEDYDTTTEFDYGDATPCQKVNERAFGAQLLPPLYSLVFVIGLVGNILVVLVLVQYKRLKNMTSIYLLNLAISDLLFLFTLPFWIDYKLKDDWVFGDAMCKILSGFYYTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIIIWALAILASMPGLYFSKTQWEFTHHTCSLHFPHESLREWKLFQALKLNLFGLVLPLLVMIICYTGIIKILLRRPNEKKSKAVRLIFVIMIIFFLFWTPYNLTILISVFQDFLFTHECEQSRHLDLAVQVTEVIAYTHCCVNPVIYAFVGERFRKYLRQLFHRRVAVHLVKWLPFLSVDRLERVSSTSPSTGEHELSAGF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Bovine
80
Sheep
80
Pig
60
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32246 基板として

Site PTM Type Enzyme
Y41 Phosphorylation
K326 Ubiquitination
S332 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S345 Phosphorylation
T346 Phosphorylation
S352 Phosphorylation

研究背景

機能:

Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in different hematopoietic cells.

サブユニット構造:

Interacts with CREB3.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). Bioinformatics Analysis for Identification of Key Genes in Salivary Gland and the Potential of a Combination of Biomarkers for the Diagnosis of SS. Journal of Inflammation Research, 2021 (PubMed: 34475773) [IF=4.5]

Application: IF/ICC    Species: Human    Sample: gland tissue

Figure 5 H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.

Application: IHC    Species: human    Sample: Labial Gland Tissues

Figure 5| H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.
2). High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. American Journal of Translational Research, 2019 (PubMed: 30788000) [IF=2.2]

Application: IHC    Species: human    Sample: primary NSCLC and bone metastasis tissues

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.

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