IRAK4 Antibody - #BF0371
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# BF0371, RRID:AB_2833937.
折りたたみ／展開
IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;
免疫原
Purified recombinant fragment of human IRAK4 expressed in E. Coli.
- Q9NWZ3 IRAK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS
PTMs - Q9NWZ3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| T6 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S8 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K33 | Ubiquitination | Uniprot | |
| K34 | Acetylation | Uniprot | |
| S114 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K115 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S151 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S152 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S157 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T209 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K313 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T342 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T345 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S346 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T352 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K400 | Sumoylation | Uniprot | |
| K416 | Ubiquitination | Uniprot |
PTMs - Q9NWZ3 酵素として
| Substrate | Site | Source |
|---|---|---|
| P14598 (NCF1) | T133 | Uniprot |
| P14598 (NCF1) | T138 | Uniprot |
| P14598 (NCF1) | T153 | Uniprot |
| P14598 (NCF1) | S288 | Uniprot |
| P14598 (NCF1) | S320 | Uniprot |
| P14598 (NCF1) | S345 | Uniprot |
| P14598 (NCF1) | S348 | Uniprot |
| P14598 (NCF1) | T356 | Uniprot |
| P14598 (NCF1) | S359 | Uniprot |
| P14598 (NCF1) | S370 | Uniprot |
| P51617-4 (IRAK1) | S376 | Uniprot |
| P51617-1 (IRAK1) | T387 | Uniprot |
| P58753 (TIRAP) | T28 | Uniprot |
| Q96FA3 (PELI1) | S70 | Uniprot |
| Q96FA3 (PELI1) | S76 | Uniprot |
| Q96FA3 (PELI1) | S78 | Uniprot |
| Q96FA3 (PELI1) | T80 | Uniprot |
| Q96FA3 (PELI1) | S82 | Uniprot |
| Q96FA3 (PELI1) | T86 | Uniprot |
| Q96FA3 (PELI1) | T127 | Uniprot |
| Q96FA3 (PELI1) | T288 | Uniprot |
| Q96FA3 (PELI1) | S293 | Uniprot |
| Q9NWZ3 (IRAK4) | T6 | Uniprot |
| Q9NWZ3 (IRAK4) | S8 | Uniprot |
| Q9NWZ3 (IRAK4) | S114 | Uniprot |
| Q9NWZ3 (IRAK4) | S150 | Uniprot |
| Q9NWZ3 (IRAK4) | S151 | Uniprot |
| Q9NWZ3 (IRAK4) | S152 | Uniprot |
| Q9NWZ3 (IRAK4) | T208 | Uniprot |
| Q9NWZ3 (IRAK4) | T209 | Uniprot |
| Q9NWZ3 (IRAK4) | T342 | Uniprot |
| Q9NWZ3 (IRAK4) | T345 | Uniprot |
| Q9NWZ3 (IRAK4) | S346 | Uniprot |
| Q9NWZ3 (IRAK4) | T352 | Uniprot |
研究背景
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Phosphorylated.
Cytoplasm.
Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome. Once phosphorylated, IRAK4 dissociates from the receptor complex and then associates with the TNF receptor-associated factor 6 (TRAF6), IRAK1, and PELI1; this intermediate complex is required for subsequent NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Interacts with IL1RL1.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
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