製品: GHSR Antibody
カタログ: DF2794
タンパク質の説明: Rabbit polyclonal antibody to GHSR
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit
分子量: 41 kDa; 41kD(Calculated).
ユニプロット: Q92847
RRID: AB_2840000

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(86%), Rabbit(86%)
クローナリティ:
Polyclonal
特異性:
GHSR Antibody detects endogenous levels of total GHSR.
RRID:
AB_2840000
引用形式: Affinity Biosciences Cat# DF2794, RRID:AB_2840000.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

GH releasing peptide receptor; GH-releasing peptide receptor; Ghrelin receptor; GHRP; GHRPR; GHS-R; GHSR; GHSR_HUMAN; Growth hormone secretagogue receptor 1; Growth hormone secretagogue receptor type 1; growth hormone secretatgogue receptor;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q92847 GHSR_HUMAN:

Pituitary and hypothalamus.

タンパク質の説明:
Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).;
タンパク質配列:
MWNATPSEEPGFNLTLADLDWDASPGNDSLGDELLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVIFVIWAVAFCSAGPIFVLVGVEHENGTDPWDTNECRPTEFAVRSGLLTVMVWVSSIFFFLPVFCLTVLYSLIGRKLWRRRRGDAVVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFRLLGFEPFSQRKLSTLKDESSRAWTESSINT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
86
Horse
86
Sheep
75
Pig
71
Bovine
71
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92847 基板として

Site PTM Type Enzyme

研究背景

機能:

Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Pituitary and hypothalamus.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

参考文献

1). Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring. Scientific Reports, 2023 (PubMed: 37355724) [IF=4.6]

Application: IHC    Species: Rat    Sample:

Figure 3 Quantitative analysis of the intensity of ghrelin IR in (A) villi and (B) crypts of small intestine segments, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the higher the intensity of the IR). (C) Representative images of the ghrelin IR in sections of proximal jejunum; inset: antibody control. Quantitative analysis of the intensity of ghrelin receptor IR in (D) villi and (E) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (F) Representative images of the ghrelin IR in sections of duodenum. (C), (F): All scale bars represent 100 µm. (A), (B), (D), (E): Bar plots show lsmeans value and standard deviation (whiskers). A p-value range was attributed above plots when two groups exhibit significant differences: *p value 

2). Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins. Animals, 2023 (PubMed: 37174575) [IF=3.0]

Application: IHC    Species: Rat    Sample:

Figure 2 Representative images and quantitative analysis of the intensity of ghrelin, ghrelin receptor, leptin, and leptin receptor immunoreaction (IR) in crypts and villi of the duodenum of female newborn rat offspring after maternal exposure to fumonisins (FBs) at the doses of 0, 60, or 90 mg/kg b.w. Bottom isotype row shows IHC IR control incubated without a primary antibody. All of the images were taken at the same magnification; scale bars represent 50 μm. Bar charts: intensity of immunoreaction (IR) to specific antibody was measured in crypts and villi using optical density (OD) measurements in the microscopic images. Results are presented as mean and SD. Statistically significant differences between groups are indicated by

3). Ghrelin inhibits early brain injury due to subarachnoid hemorrhage via the Tim-3-mediated HMGB1/NF-κB pathway. JOURNAL OF CHEMICAL NEUROANATOMY, 2022 (PubMed: 35863561) [IF=2.8]

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