Cytochrome C Antibody | Affinity Biosciences

製品:	Cytochrome C Antibody
カタログ:	AF0146
タンパク質の説明:	Rabbit polyclonal antibody to Cytochrome C
アプリケーション:	WB IHC IF/ICC
反応性:	Human, Mouse, Rat, Monkey
予測:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量:	15kDa; 12kD(Calculated).
ユニプロット:	P99999
RRID:	AB_2833328

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat,Monkey

予測:

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

クローナリティ:

Polyclonal

特異性:

Cytochrome C Antibody detects endogenous levels of total Cytochrome C.

RRID:

AB_2833328
引用形式: Affinity Biosciences Cat# AF0146, RRID:AB_2833328.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

CYC; CYC_HUMAN; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4;

免疫原

免疫原:

Uniprot:

P99999(CYC_HUMAN) >>Visit HPA database.

遺伝子(ID):

CYCS(54205)

タンパク質の説明:

CYCS Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

タンパク質配列:

P99999 CYC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P99999 基板として

P99999

Site	PTM Type	Source
G2	Acetylation	Uniprot
K9	Acetylation	Uniprot
K28	Sumoylation	Uniprot
K28	Ubiquitination	Uniprot
T29	Phosphorylation	Uniprot
K40	Ubiquitination	Uniprot
T41	Phosphorylation	Uniprot
Y47	Phosphorylation	Uniprot
S48	Phosphorylation	Uniprot
Y49	Phosphorylation	Uniprot
T50	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
Y68	Phosphorylation	Uniprot
K73	Acetylation	Uniprot
K73	Ubiquitination	Uniprot
K74	Acetylation	Uniprot
K74	Ubiquitination	Uniprot
Y75	Phosphorylation	Uniprot
T79	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
K87	Acetylation	Uniprot
K87	Ubiquitination	Uniprot
K88	Acetylation	Uniprot
K89	Acetylation	Uniprot
Y98	Phosphorylation	Uniprot
K100	Acetylation	Uniprot
K100	Methylation	Uniprot
K100	Ubiquitination	Uniprot
K101	Methylation	Uniprot

研究背景

P99999_CYC_HUMAN

機能:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

PTMs:

Binds 1 heme group per subunit.

Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

細胞の位置付け:

Mitochondrion intermembrane space.
Note: Loosely associated with the inner membrane.

タンパク質ファミリー:

Belongs to the cytochrome c family.

研究領域

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Metabolism > Energy metabolism > Sulfur metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Reversible Deformation of Artificial Cell Colonies Triggered by Actin Polymerization for Muscle Behavior Mimicry. Advanced materials (Deerfield Beach, Fla.), 2022 (PubMed: 35765153) [IF=29.4]

2). Synthesis of hydroxycinnamic acid derivatives as mitochondria-targeted antioxidants and cytotoxic agents. Acta Pharmaceutica Sinica B, 2017 (PubMed: 28119815) [IF=14.5]

Application: WB Species: Human Sample:

Figure 7 Detection of cyt c release from energized mitochondria suspensions (2 mg protein/mL). (A) In the presence of 10 μmol/L MitoHCAs. a: control; b: MitoCaA; c: MitoFA; d: Mitop-CoA; e: MitoCA. (B) a: control; b: 1 μmol/L MitoCaA; c: 20 μmol/L MitoCaA +1 μmol/L CsA; d: 20 μmol/L MitoCaA. The blots shown are representative of three independent experiments demonstrating similar results. (C) In the presence of the various concentrations of MitoCaA. (D) In the presence of MitoHCAs, CaA and butylTPP (10 μmol/L) with or without Ca2+. The quantitative assays were performed by the dithionite-reduction method. The values represent mean±SEM of three independent experiments. ***P

3). Acceleration mechanism of riboflavin on Fe0-to-microbe electron transfer in corrosion of EH36 steel by Pseudomonas aeruginosa. The Science of the total environment, 2024 (PubMed: 38815822) [IF=9.8]

4). Inhibition of fatty acid catabolism augments the efficacy of oxaliplatin-based chemotherapy in gastrointestinal cancers. CANCER LETTERS, 2020 (PubMed: 31904482) [IF=9.7]

5). Iron induces B cell pyroptosis through Tom20–Bax–caspase–gasdermin E signaling to promote inflammation post-spinal cord injury. Journal of Neuroinflammation, 2023 (PubMed: 37480037) [IF=9.3]

Application: WB Species: Mouse Sample: B cells

Fig. 3 Pyroptosis induced by iron accumulation may occur in splenic B cells after SCI. a Three days after SCI, serum samples were collected for quantification of the protein expression levels of MCP-1, IL-1β, IL-6, and TNF-α by ELISA. b Three days after SCI, injured spinal cord homogenates were collected for quantification of the protein expression levels of MCP-1, IL-1β, IL-6, and TNF-α by ELISA. c Three days after SCI, the spleen was stained with Prussian blue to detect the level of iron ion. d Three days after SCI, serum samples were collected to quantify the concentration of iron ions. e Three days after SCI, B cell lysates were collected to quantify the concentration of iron ions. f Detection of Tom20-Bax-caspase-GSDME pathway-related protein expression in B cells by western blot. g The knock-down efficiency of AAV on Tom20 in B cells was verified. h, l, m The expression levels of MCP-1, IL-1 β, IL-6, TNF- α, IgG and IgM in serum of different groups were detected by ELISA. i–k Three days after SCI, the spleens of mice were observed, and the spleen length and organ index of different groups were compared. n, o, q The spleen was taken for HE staining, Prussian blue staining, and immunofluorescence. CD19+ was red, dapi was blue, and merge was combined. p Detection of nerve evoked potentials in different groups of mice. r Detection of the expression of Tom20-Bax-caspsae-GSDME pathway-related proteins in different groups of B cells. All data are expressed as the mean ± SD (n ≥ 3 replicates per group). ns P > 0.05, * P

6). The anti-hepatocellular carcinoma effect of Brucea javanica oil in ascitic tumor-bearing mice: The detection of brusatol and its role. Biomedicine & Pharmacotherapy, 2021 (PubMed: 33341052) [IF=7.5]

7). Emodin targets mitochondrial cyclophilin D to induce apoptosis in HepG2 cells. BIOMEDICINE & PHARMACOTHERAPY, 2017 (PubMed: 28363167) [IF=7.5]

Application: WB Species: human Sample:

Fig. 2. Effects of CsA on emodin-induced apoptosis. The cells were treated with emodin for 48 h in the presence or absence of CsA (5mM), then assays were performed. (A) Analysis of apoptosis by nuclear condensation. The Hoechst 33342 staining showed typical apoptotic morphology changes after emodin treatment. The images were acquired by inverted fluorescence microscopy. (B) Analysis of apoptosis by Annexin V/PI double-staining assay. (C) Determination of Cyto-C level in mitochondria and cytosol by western blots. b-actin and VDAC1 were used as internal control.

8). Edaravone combined with dexamethasone exhibits synergic effects on attenuating smoke-induced inhalation lung injury in rats. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34225014) [IF=7.5]

9). Proteus mirabilis Vesicles Induce Mitochondrial Apoptosis by Regulating miR96-5p/Abca1 to Inhibit Osteoclastogenesis and Bone Loss. Frontiers in Immunology, 2022 (PubMed: 35242136) [IF=7.3]

Application: WB Species: mouse Sample: Osteoclasts

FIGURE 4 | Effect of P.M OMVs on mitochondria-dependent osteoclast apoptosis.(J) Cyto c expression in the cytoplasm and mitochondria with or without P.M OMV treatment. Data were obtained from three independent experiments and represented as mean ± SD. ####p < 0.0001 compared to M-CSF. *p < 0.05; **p < 001; ***p < 0.001; ****p < 0.0001 compared to M-CSF + RANKL. ns, not significant.

Application: WB Species: Mouse Sample: osteoclasts

Figure 4 Effect of P.M OMVs on mitochondria-dependent osteoclast apoptosis. (A) Venn diagram showing the intersection of negatively correlated miRNA–mRNA pairs within miRNA and mRNA sequences. (B) KEGG enrichment analysis of upregulated genes is shown in the bubble chart. The top 15 genes significantly enriched in the KEGG pathway (p < 0.05) are presented. Changes in osteoclast (C, D) apoptosis, (E, F) intracellular ROS, and (G, H) mitochondrial membrane potential level after being cultured with or without 0.15 or 0.3 μg/ml of P.M OMVs for 5 days. (I) Western blot analysis of caspase-3, Bcl-2, and Bax. (J) Cyto c expression in the cytoplasm and mitochondria with or without P.M OMV treatment. Data were obtained from three independent experiments and represented as mean ± SD. ####p < 0.0001 compared to M-CSF. *p < 0.05; **p < 001; ***p < 0.001; ****p < 0.0001 compared to M-CSF + RANKL. ns, not significant.

10). Fraxetin pretreatment alleviates cisplatin-induced kidney injury by antagonizing autophagy and apoptosis via mTORC1 activation. Phytotherapy research : PTR, 2024 (PubMed: 38558449) [IF=7.2]

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Cytochrome C Antibody - #AF0146

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