NEFL Antibody - #BF0117
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# BF0117, RRID:AB_2833978.
折りたたみ／展開
150kDa medium; 160 kDa neurofilament protein; 2 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; AI847934; CMT1F; CMT2CC; CMT2E; FLJ53642; KIAA0845; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; mKIAA0845; Nef3; NEFH; NEFL; NEFM; Neurofilament 3; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament medium polypeptide 150kDa; Neurofilament medium polypeptide; Neurofilament protein heavy polypeptide; Neurofilament protein light chain; Neurofilament protein light polypeptide; Neurofilament protein medium polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; NFM_HUMAN; PPP1R110; protein phosphatase 1, regulatory subunit 110;
免疫原
Purified recombinant fragment of human NEFL expressed in E. Coli.
- P07196 NFL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
PTMs - P07196 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| T21 | Phosphorylation | Q00535 (CDK5) , P28482 (MAPK1) | Uniprot |
| R23 | Methylation | Uniprot | |
| R30 | Methylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K362 | Acetylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| K379 | Acetylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| T520 | Phosphorylation | Uniprot |
研究背景
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.
Interacts with ARHGEF28. Interacts with TRIM2.
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Belongs to the intermediate filament family.
研究領域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
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