E2F4 Antibody - #AF0151
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0151, RRID:AB_2833333.
折りたたみ／展開
E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; E2F-4; E2F4; E2F4_HUMAN; p107/p130 binding protein; Transcription factor E2F 4; Transcription factor E2F4; E2F 5; E2F transcription factor 5; E2F transcription factor 5 p130 binding; E2F-5; E2f5; E2F5_HUMAN; p130 binding; Transcription factor E2F5;
免疫原
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Q16254 E2F4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL
- Q15329 E2F5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAEPASSGQQAPAGQGQGQRPPPQPPQAQAPQPPPPPQLGGAGGGSSRHEKSLGLLTTKFVSLLQEAKDGVLDLKAAADTLAVRQKRRIYDITNVLEGIDLIEKKSKNSIQWKGVGAGCNTKEVIDRLRYLKAEIEDLELKERELDQQKLWLQQSIKNVMDDSINNRFSYVTHEDICNCFNGDTLLAIQAPSGTQLEVPIPEMGQNGQKKYQINLKSHSGPIHVLLINKESSSSKPVVFPVPPPDDLTQPSSQSLTPVTPQKSSMATQNLPEQHVSERSQALQQTSATDISSAGSISGDIIDELMSSDVFPLLRLSPTPADDYNFNLDDNEGVCDLFDVQILNY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16254/Q15329 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T248 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K53 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot |
研究背景
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Differentially phosphorylated in vivo.
Nucleus.
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. The E2F4/TFDP1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with CEBPA (when phosphorylated).
Belongs to the E2F/DP family.
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Nucleus.
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.
Belongs to the E2F/DP family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
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