STAT5A Antibody - #BF0022

(1)  
製品: STAT5A Antibody
カタログ: BF0022
タンパク質の説明: Mouse monoclonal antibody to STAT5A
アプリケーション: WB IHC IF/ICC ELISA FACS
反応性: Human
分子量: 90.6kDa; 91kD(Calculated).
ユニプロット: P42229
RRID: AB_2834061

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFB2039]
特異性:
STAT5A antibody detects endogenous levels of total STAT5A.
RRID:
AB_2834061
引用形式: Affinity Biosciences Cat# BF0022, RRID:AB_2834061.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Mammary gland factor; MGF; Signal transducer and activator of transcription 5A; STA5A_HUMAN; STAT 5; STAT 5A; STAT5; STAT5A;

免疫原

免疫原:

Purified recombinant fragment of human STAT5A expressed in E. Coli.

Uniprot:

P42229(STA5A_HUMAN) >>Visit HPA database.

遺伝子(ID):
STAT5A(6776)
タンパク質の説明:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
タンパク質配列:
MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS

PTMs - P42229 基板として

Site PTM Type Enzyme
Y22 Phosphorylation
K70 Ubiquitination
K71 Ubiquitination
K84 Acetylation
K84 Ubiquitination
K86 Ubiquitination
Y90 Phosphorylation
T92 O-Glycosylation
T92 Phosphorylation
Y114 Phosphorylation
S127 Phosphorylation
S128 Phosphorylation
K163 Ubiquitination
K164 Ubiquitination
S193 Phosphorylation
K207 Ubiquitination
K232 Ubiquitination
K235 Ubiquitination
K242 Ubiquitination
T245 Phosphorylation
K256 Sumoylation
K256 Ubiquitination
K336 Sumoylation
K343 Ubiquitination
K359 Ubiquitination
K370 Ubiquitination
T372 Phosphorylation
S375 Phosphorylation
K380 Ubiquitination
K384 Acetylation
K384 Sumoylation
K384 Ubiquitination
S473 Phosphorylation
K516 Sumoylation
K516 Ubiquitination
K527 Ubiquitination
K586 Ubiquitination
K644 Ubiquitination
T647 Phosphorylation
T648 Phosphorylation
Y668 Phosphorylation
K675 Ubiquitination
K681 Ubiquitination
Y682 Phosphorylation
Y683 Phosphorylation
K689 Ubiquitination
Y694 Phosphorylation Q06187 (BTK) , P12931 (SRC) , P52333 (JAK3) , P06239 (LCK) , P06213 (INSR) , P07948 (LYN) , P43405 (SYK) , Q13882 (PTK6) , O60674 (JAK2) , P08631 (HCK)
K696 Acetylation
K696 Sumoylation
K696 Ubiquitination
K700 Acetylation
Y720 Phosphorylation
S726 Phosphorylation
T757 Phosphorylation
S774 Phosphorylation
S777 Phosphorylation
S780 Phosphorylation Q13153 (PAK1) , P28482 (MAPK1) , P27361 (MAPK3)
S792 Phosphorylation
S794 Phosphorylation

研究背景

機能:

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

PTMs:

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694.

ISGylated.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7. Interacts with ERBB4.

タンパク質ファミリー:

Belongs to the transcription factor STAT family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

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