TWIST1 Antibody - #BF0018
| 製品: | TWIST1 Antibody |
| カタログ: | BF0018 |
| タンパク質の説明: | Mouse monoclonal antibody to TWIST1 |
| アプリケーション: | WB ELISA |
| 反応性: | Human |
| 分子量: | 21kDa; 21kD(Calculated). |
| ユニプロット: | Q15672 |
| RRID: | AB_2834065 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# BF0018, RRID:AB_2834065.
折りたたみ／展開
ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; TWIST; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; TWIST1; TWST1_HUMAN;
免疫原
Purified recombinant fragment of human TWIST1 expressed in E. Coli.
- Q15672 TWST1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH
PTMs - Q15672 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S18 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S20 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K38 | Ubiquitination | Uniprot | |
| S42 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S45 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Q15759 (MAPK11) , P45983 (MAPK8) , Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K73 | Acetylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T137 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot |
研究背景
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
Nucleus.
Subset of mesodermal cells.
Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).
研究領域
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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