Phospho-EPS15 (Tyr849) Antibody - #AF0058
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0058, RRID:AB_2834101.
折りたたみ／展開
AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15;
免疫原
A synthesized peptide derived from human EPS15 around the phosphorylation site of Tyr849.
- P42566 EPS15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA
PTMs - P42566 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K227 | Methylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K234 | Methylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K357 | Acetylation | Uniprot | |
| K359 | Acetylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K648 | Ubiquitination | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| T663 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| K774 | Ubiquitination | Uniprot | |
| T777 | Phosphorylation | Uniprot | |
| T779 | Phosphorylation | Uniprot | |
| K788 | Acetylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| S790 | Phosphorylation | Uniprot | |
| K793 | Ubiquitination | Uniprot | |
| S796 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K801 | Ubiquitination | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| K816 | Ubiquitination | Uniprot | |
| K818 | Ubiquitination | Uniprot | |
| K836 | Ubiquitination | Uniprot | |
| Y849 | Phosphorylation | P00533 (EGFR) | Uniprot |
| Y850 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| K890 | Ubiquitination | Uniprot |
研究背景
Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.
Ubiquitinated.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Clathrin-coated pit.
Note: Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.
Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Colocalizes with HGS on bilayered clathrin coats on endosomes.
Ubiquitously expressed.
Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity).
(Microbial infection) Interacts with vaccinia virus protein A36.
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
The UIM (ubiquitin-interacting motif) repeats specifically bind 'Lys-33'-linked ubiquitin.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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