Phospho-HCLS1 (Tyr378) Antibody - #AF0063
| 製品: | Phospho-HCLS1 (Tyr378) Antibody |
| カタログ: | AF0063 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-HCLS1 (Tyr378) |
| アプリケーション: | WB |
| 反応性: | Human |
| 分子量: | 78kDa; 54kD(Calculated). |
| ユニプロット: | P14317 |
| RRID: | AB_2834105 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0063, RRID:AB_2834105.
折りたたみ／展開
Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; HS1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75;
免疫原
- P14317 HCLS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGTRGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVAVYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE
PTMs - P14317 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T23 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S32 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K123 | Acetylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| R155 | Methylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | P07948 (LYN) , P09769 (FGR) | Uniprot |
| K223 | Acetylation | Uniprot | |
| K224 | Acetylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K241 | Acetylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| Y378 | Phosphorylation | P07948 (LYN) , P43405 (SYK) | Uniprot |
| Y397 | Phosphorylation | P09769 (FGR) , P07948 (LYN) , P43405 (SYK) | Uniprot |
| Y481 | Phosphorylation | Uniprot |
研究背景
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.
Membrane>Peripheral membrane protein. Cytoplasm. Mitochondrion.
Expressed only in tissues and cells of hematopoietic origin.
Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS (By similarity). Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54 (By similarity).
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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