MMP3 Antibody - #AF0217

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製品: MMP3 Antibody
カタログ: AF0217
タンパク質の説明: Rabbit polyclonal antibody to MMP3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Horse, Sheep, Rabbit, Dog
分子量: 53kDa; 54kD(Calculated).
ユニプロット: P08254
RRID: AB_2833347

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)
クローナリティ:
Polyclonal
特異性:
MMP3 Antibody detects endogenous levels of total MMP3.
RRID:
AB_2833347
引用形式: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

免疫原

免疫原:
Uniprot:

P08254(MMP3_HUMAN) >>Visit HPA database.

遺伝子(ID):
MMP3(4314)
タンパク質の説明:
MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.
タンパク質配列:
MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
93
Rabbit
92
Sheep
87
Xenopus
55
Pig
36
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08254 基板として

Site PTM Type Enzyme
Y18 Phosphorylation
Y116 Phosphorylation
Y121 Phosphorylation
K127 Ubiquitination

研究背景

機能:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究領域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE, 2021 (PubMed: 34371251) [IF=7.9]

Application: WB    Species: Rat    Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC    Species: Rat    Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.
2). Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. Phytomedicine, 2022 (PubMed: 36162242) [IF=7.9]
3). Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE, 2022 (PubMed: 35905566) [IF=7.9]
4). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=7.5]

Application: WB    Species: Rat    Sample:

Fig. 3. VK2 rescues rat primary chondrocytes from TBHP-Induced cell death and ECM degradation. (A) The working concentration of VK in vitro was screened by CCK8; (B) Flow cytometry detection of apoptosis; (C) Flow cytometry statistics for detection of apoptosis; (D) Representative pictures of chondrocytes of toluidine blue staining (Scale bar=200μm); (E-J) WB results for ACAN, COL2A1, SOX9, MMP3, MMP13 and Tubulin.
5). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36379122) [IF=7.5]
6). Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34311530) [IF=7.5]
7). White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy. British journal of pharmacology, 2024 (PubMed: 38644540) [IF=7.3]
8). A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage. British journal of pharmacology, 2022 (PubMed: 35731978) [IF=7.3]
9). Quercetin-3-O-β-D-glucuronide attenuates osteoarthritis by inhibiting cartilage extracellular matrix degradation and inflammation. Journal of orthopaedic translation, 2024 (PubMed: 38601200) [IF=6.6]
10). TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. International Journal of Cancer, 2017 (PubMed: 28670703) [IF=6.4]

Application: IHC    Species: human    Sample: GCTB

Supplementary Figure 1 | The expression of MMP3 in GCT of bone. The expression level of MMP3 showed no significant correlation with TGF-β and PAR-1 expression.
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