製品: Hsp40 Antibody
カタログ: AF0586
タンパク質の説明: Rabbit polyclonal antibody to Hsp40
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Dog
分子量: 45kDa; 38kD(Calculated).
ユニプロット: P25685
RRID: AB_2834148

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Hsp40 Antibody detects endogenous levels of total Hsp40.
RRID:
AB_2834148
引用形式: Affinity Biosciences Cat# AF0586, RRID:AB_2834148.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DnaJ (Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
DNAJB1 Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Interacts with DNAJC3. Interacts with SRPK1. By heat shock. Note: This description may include information from UniProtKB.
タンパク質配列:
MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25685 基板として

Site PTM Type Enzyme
K3 Ubiquitination
Y5 Phosphorylation
R13 Methylation
S16 Phosphorylation
K21 Ubiquitination
K35 Ubiquitination
K37 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K46 Ubiquitination
Y52 Phosphorylation
K60 Ubiquitination
R66 Methylation
T88 Phosphorylation
S89 Phosphorylation
S149 Phosphorylation Q8IW41 (MAPKAPK5)
S151 Phosphorylation Q8IW41 (MAPKAPK5)
K159 Ubiquitination
S171 Phosphorylation Q8IW41 (MAPKAPK5)
Y176 Phosphorylation
S177 Phosphorylation
T180 Phosphorylation
K181 Acetylation
K181 Ubiquitination
S186 Phosphorylation
K195 Acetylation
K195 Ubiquitination
S196 Phosphorylation
K202 Ubiquitination
T205 Phosphorylation
K217 Ubiquitination
K222 Ubiquitination
T227 Phosphorylation
S228 Phosphorylation
K240 Ubiquitination
K242 Ubiquitination
K248 Ubiquitination
S252 Phosphorylation
Y256 Phosphorylation
S261 Phosphorylation
K286 Ubiquitination
K296 Ubiquitination
K306 Ubiquitination
T307 Phosphorylation

研究背景

機能:

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).

細胞の位置付け:

Cytoplasm. Nucleus. Nucleus>Nucleolus.
Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with DNAJC3. Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

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