NCK2 Antibody - #AF0679
| 製品: | NCK2 Antibody |
| カタログ: | AF0679 |
| タンパク質の説明: | Rabbit polyclonal antibody to NCK2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 42kDa; 43kD(Calculated). |
| ユニプロット: | O43639 |
| RRID: | AB_2834155 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0679, RRID:AB_2834155.
折りたたみ／展開
Cytoplasmic protein NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta;
免疫原
- O43639 NCK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43639 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y342 | Phosphorylation | Uniprot |
研究背景
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Phosphorylated.
Cytoplasm. Endoplasmic reticulum.
Ubiquitous.
Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.
研究領域
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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