NKX3.1 Antibody - #AF0221
| 製品: | NKX3.1 Antibody |
| カタログ: | AF0221 |
| タンパク質の説明: | Rabbit polyclonal antibody to NKX3.1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Horse, Rabbit, Dog |
| 分子量: | 38kDa; 26kD(Calculated). |
| ユニプロット: | Q99801 |
| RRID: | AB_2834156 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0221, RRID:AB_2834156.
折りたたみ／展開
BAPX 2; BAPX2; Homeobox protein NK-3 homolog A; Homeobox protein Nkx 3.1; Homeobox protein Nkx-3.1; Homeobox protein Nkx3.1; NK homeobox (Drosophila) family 3 A; NK homeobox family 3 A; NK homeobox, family 3, member A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related locus 1; NKX 3; Nkx 3.1; NKX 3A; NKX3 1; NKX3; Nkx3-1; NKX3.1; Nkx3.1, mouse, homolog of; NKX31_HUMAN; NKX3A;
免疫原
- Q99801 NKX31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRVPEPRPGEAKAEGAAPPTPSKPLTSFLIQDILRDGAQRQGGRTSSQRQRDPEPEPEPEPEGGRSRAGAQNDQLSTGPRAAPEEAETLAETEPERHLGSYLLDSENTSGALPRLPQTPKQPQKRSRAAFSHTQVIELERKFSHQKYLSAPERAHLAKNLKLTETQVKIWFQNRRYKTKRKQLSSELGDLEKHSSLPALKEEAFSRASLVSVYNSYPYYPYLYCVGSWSPAFW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99801 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T21 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T89 | Phosphorylation | P11309 (PIM1) , P19784 (CSNK2A2) | Uniprot |
| T93 | Phosphorylation | P19784 (CSNK2A2) | Uniprot |
| T134 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K159 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K182 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | P11309 (PIM1) , Q9Y463 (DYRK1B) | Uniprot |
| S186 | Phosphorylation | P11309 (PIM1) | Uniprot |
| S195 | Phosphorylation | P11309 (PIM1) | Uniprot |
| S196 | Phosphorylation | P11309 (PIM1) | Uniprot |
| Y222 | Phosphorylation | Uniprot |
研究背景
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
Nucleus.
Highly expressed in the prostate and, at a lower level, in the testis.
Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation.
Belongs to the NK-3 homeobox family.
研究領域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
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