SENP6 Antibody - #AF0277

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製品: SENP6 Antibody
カタログ: AF0277
タンパク質の説明: Rabbit polyclonal antibody to SENP6
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 126kDa; 126kD(Calculated).
ユニプロット: Q9GZR1
RRID: AB_2834163

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
SENP6 Antibody detects endogenous levels of total SENP6.
RRID:
AB_2834163
引用形式: Affinity Biosciences Cat# AF0277, RRID:AB_2834163.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2810017C20Rik; FLJ11355; FLJ11887; KIAA0389; KIAA0797; SENP 6; Senp6; SENP6_HUMAN; Sentrin specific protease 6; Sentrin-specific protease 6; Sentrin/SUMO specific protease SENP6; Sentrin/SUMO-specific protease SENP6; SSP 1; SSP1; SUMO 1 specific protease 1; SUMO 1 specific protease; SUMO-1-specific protease 1; SUMO1 specific protease 1; SUMO1 specific protease; SUMO1/sentrin specific peptidase 6; SUSP 1; SUSP1;

免疫原

免疫原:
Uniprot:

Q9GZR1(SENP6_HUMAN) >>Visit HPA database.

遺伝子(ID):
SENP6(26054)
発現特異性:
Q9GZR1 SENP6_HUMAN:

Highly expressed in reproductive organs, such as testis, ovary and prostate.

タンパク質の説明:
SENP6 Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Does not seem to be involved in the processing of full-length SUMO proteins to their mature forms. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. May act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties. Belongs to the peptidase C48 family.
タンパク質配列:
MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDEDSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNMLSNNKKLSENTQNTSLCSGTVVHGRRFHHAHAQIPVVKTAAQSSLDRKERKEYPPHVQKVEINPVRLSRLQGVERIMKKTEESESQVEPEIKRKVQQKRHCSTYQPTPPLSPASKKCLTHLEDLQRNCRQAITLNESTGPLLRTSIHQNSGGQKSQNTGLTTKKFYGNNVEKVPIDIIVNCDDSKHTYLQTNGKVILPGAKIPKITNLKERKTSLSDLNDPIILSSDDDDDNDRTNRRESISPQPADSACSSPAPSTGKVEAALNENTCRAERELRSIPEDSELNTVTLPRKARMKDQFGNSIINTPLKRRKVFSQEPPDALALSCQSSFDSVILNCRSIRVGTLFRLLIEPVIFCLDFIKIQLDEPDHDPVEIILNTSDLTKCEWCNVRKLPVVFLQAIPAVYQKLSIQLQMNKEDKVWNDCKGVNKLTNLEEQYIILIFQNGLDPPANMVFESIINEIGIKNNISNFFAKIPFEEANGRLVACTRTYEESIKGSCGQKENKIKTVSFESKIQLRSKQEFQFFDEEEETGENHTIFIGPVEKLIVYPPPPAKGGISVTNEDLHCLNEGEFLNDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQRERRNHETTNLSIQQKRHGRVKTWTRHVDIFEKDFIFVPLNEAAHWFLAVVCFPGLEKPKYEPNPHYHENAVIQKCSTVEDSCISSSASEMESCSQNSSAKPVIKKMLNKKHCIAVIDSNPGQEESDPRYKRNICSVKYSVKKINHTASENEEFNKGESTSQKVADRTKSENGLQNESLSSTHHTDGLSKIRLNYSDESPEAGKMLEDELVDFSEDQDNQDDSSDDGFLADDNCSSEIGQWHLKPTICKQPCILLMDSLRGPSRSNVVKILREYLEVEWEVKKGSKRSFSKDVMKGSNPKVPQQNNFSDCGVYVLQYVESFFENPILSFELPMNLANWFPPPRMRTKREEIRNIILKLQEDQSKEKRKHKDTYSTEAPLGEGTEQYVNSISD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZR1 基板として

Site PTM Type Enzyme
S6 Phosphorylation
S9 Phosphorylation
S22 Phosphorylation
S35 Phosphorylation
S42 Phosphorylation
S55 Phosphorylation
K99 Methylation
T100 Phosphorylation
K102 Methylation
K189 Sumoylation
Y214 Phosphorylation
T217 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation
K226 Ubiquitination
K264 Ubiquitination
K274 Sumoylation
K319 Ubiquitination
T323 Phosphorylation
S335 Phosphorylation
S336 Phosphorylation
T345 Phosphorylation
S350 Phosphorylation
S352 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation
T398 Phosphorylation
K406 Sumoylation
S412 Phosphorylation
T416 Phosphorylation
K419 Sumoylation
K534 Ubiquitination
S577 Phosphorylation
Y599 Phosphorylation
S606 Phosphorylation
K736 Ubiquitination
S819 Phosphorylation
S869 Phosphorylation
K910 Ubiquitination
S916 Phosphorylation
S919 Phosphorylation
S985 Phosphorylation
K1086 Ubiquitination
S1111 Phosphorylation

研究背景

機能:

Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in reproductive organs, such as testis, ovary and prostate.

サブユニット構造:

Interacts with RXRA. Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation. Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation.

タンパク質ファミリー:

Belongs to the peptidase C48 family.

参考文献

1). SENP6 restricts the IFN-I-induced signaling pathway and antiviral activity by deSUMOylating USP8. Cellular & molecular immunology, 2024 (PubMed: 38906982) [IF=24.1]
2). SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane Transport in Angiogenesis. International Journal of Molecular Sciences, 2023 (PubMed: 36768878) [IF=5.6]

Application: WB    Species: Human    Sample: HUVECs

Figure 3 SENP6−reduced HUVECs attenuate AGE−induced angiogenic signaling. (A) Heat map of mRNA showing the differences in SENPs expression between the control and AGEs groups. “Red” indicates up-regulation and “Green” indicates doregulation. ** indicates that p < 0.01 and *** indicates that p < 0.001 between the indicated groups. (B) Detection of SENP6 mRNA expression by qPCR at 0, 4, 8, and 12 h of AGE−stimulated HUVECs, n = 4, * p < 0.05 vs. 0 h. (C) The temporal effect of AGE stimulation on HUVECs was investigated using Western blot, which showed SENP6 protein expression at 0, 4, 8, and 12 h. (D) Analysis of protein expression level results, n = 6, * p < 0.05 vs. 0 h. AGE−induced angiogenic responses were inhibited by SENP6 silencing (Supplemental Figure S1C). HUVECs were transfected with NC siRNA or SENP6 siRNA for 48 h. Cells were subjected to HUVECs migration and tube formation in response to AGEs. (E) Transwell migration assay for the migration ability. (F) Analysis of migration assay results, n = 3, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. (G) HUVECs tube formation in a Matrigel assay. Representative images are shown in (G). (H,I) Analysis of total tube length and total branching points results, n = 4, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm (J) HUVECs migration by a scratch assay for indicated times. (K) Wound healing (% closure) was quantified, n = 5, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. * and # indicate significance between the indicated groups.

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