TNFSF14 Antibody - #AF0329

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製品: TNFSF14 Antibody
カタログ: AF0329
タンパク質の説明: Rabbit polyclonal antibody to TNFSF14
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Dog
分子量: 26kDa; 26kD(Calculated).
ユニプロット: O43557
RRID: AB_2834173

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Dog(83%)
クローナリティ:
Polyclonal
特異性:
TNFSF14 Antibody detects endogenous levels of total TNFSF14.
RRID:
AB_2834173
引用形式: Affinity Biosciences Cat# AF0329, RRID:AB_2834173.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD 258; CD258; CD258 antigen; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14; TNF14_HUMAN; TNFSF 14; Tnfsf14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor (ligand) superfamily, member 14; Tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily, member 14; Tumor necrosis factor receptor like 2; tumor necrosis factor receptor-like 2; Tumor necrosis factor superfamily member 14; Tumor necrosis factor superfamily member LIGHT;

免疫原

免疫原:
Uniprot:

O43557(TNF14_HUMAN) >>Visit HPA database.

遺伝子(ID):
TNFSF14(8740)
発現特異性:
O43557 TNF14_HUMAN:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

タンパク質の説明:
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
タンパク質配列:
MEESVVRPSVFVVDGQTDIPFTRLGRSHRRQSCSVARVGLGLLLLLMGAGLAVQGWFLLQLHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Rabbit
70
Horse
60
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43557 基板として

Site PTM Type Enzyme
S32 Phosphorylation
S34 Phosphorylation
T161 Phosphorylation
T163 Phosphorylation
Y167 Phosphorylation
T170 Phosphorylation

研究背景

機能:

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.

PTMs:

N-glycosylated.

The soluble form of isoform 1 derives from the membrane form by proteolytic processing.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein.

Secreted.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

サブユニット構造:

Homotrimer. Interacts with TNFRSF14.

タンパク質ファミリー:

Belongs to the tumor necrosis factor family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

参考文献

1). Hepatic stellate cell mediates transcription of TNFSF14 in hepatocellular carcinoma cells via H2S/CSE-JNK/JunB signaling pathway. Cell Death & Disease, 2022 (PubMed: 35292636) [IF=9.0]

Application: WB    Species: Human    Sample: HCC cells

Fig. 3 H2S upregulates pro-apoptotic factor TNFSF14 expression in HCC cells. A The heatmap. B Venn diagram of gene predictions based on the control group and HepG2 cells treated with NaHS and co-cultured with LX-2 cells. C The expression levels of TNFSF14 mRNA changed in HepG2 cells treated with NaHS and co-cultured with LX-2 cells. D RT-qPCR and (E) Western blotting were performed to examine the effects of various concentrations of NaHS on expression of TNFSF14 gene in HepG2 and PLC/PRF/5 cells. F RT-qPCR and (G) Western blotting analysis of the expression of TNFSF14 under 10−3 M NaHS treatment with different time in HepG2 and PLC/PRF/5 cells. H RT-qPCR and (I) Western blotting were used to detect the effects of PPG treated on TNFSF14 expression in HCC cells. All Bars, ± SD; n = 3; *P < 0.05 compared with control in HepG2 cells, #P < 0.05 compared with control in PLC/PRF/5 cells, &P < 0.05 compared with the co-culture group in HCC cells.

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