MED14 Antibody - #AF0336
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0336, RRID:AB_2834176.
折りたたみ／展開
Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; DRIP150; EXLM1; hRGR1; MED14; MED14_HUMAN; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; RGR1; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; Transcriptional coactivator CRSP150; Trap170; Vitamin D3 receptor-interacting protein complex 150 kDa component;
免疫原
- O60244 MED14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60244 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K111 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K399 | Methylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| Y901 | Phosphorylation | Uniprot | |
| Y927 | Phosphorylation | Uniprot | |
| Y941 | Phosphorylation | Uniprot | |
| K948 | Ubiquitination | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S1061 | Phosphorylation | Uniprot | |
| S1112 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| S1119 | Phosphorylation | Uniprot | |
| R1123 | Methylation | Uniprot | |
| S1128 | Phosphorylation | Uniprot | |
| S1133 | Phosphorylation | Uniprot | |
| S1136 | Phosphorylation | Uniprot | |
| S1142 | Phosphorylation | Uniprot | |
| S1144 | Phosphorylation | Uniprot | |
| R1146 | Methylation | Uniprot | |
| S1151 | Phosphorylation | Uniprot | |
| K1256 | Ubiquitination | Uniprot | |
| K1264 | Ubiquitination | Uniprot | |
| K1274 | Ubiquitination | Uniprot | |
| K1283 | Ubiquitination | Uniprot | |
| K1295 | Ubiquitination | Uniprot | |
| K1304 | Ubiquitination | Uniprot | |
| T1354 | Phosphorylation | Uniprot | |
| K1360 | Ubiquitination | Uniprot | |
| S1361 | Phosphorylation | Uniprot | |
| K1420 | Ubiquitination | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Nucleus.
Ubiquitous.
Interacts with GATA1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR, ESR1, SREBF1 and STAT2.
Belongs to the Mediator complex subunit 14 family.
研究領域
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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