GANP Antibody - #AF0165

(6)  
製品: GANP Antibody
カタログ: AF0165
タンパク質の説明: Rabbit polyclonal antibody to GANP
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 218kDa; 218kD(Calculated).
ユニプロット: O60318
RRID: AB_2833358

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(80%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
GANP Antibody detects endogenous levels of total GANP.
RRID:
AB_2833358
引用形式: Affinity Biosciences Cat# AF0165, RRID:AB_2833358.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

80 kDa MCM3 associated protein; 80 kDa MCM3-associated protein; FLJ44336; FLJ45306; Germinal center associated nuclear protein; KIAA0572; MAP80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; MCM3A; MCM3A_HUMAN; Mcm3ap; Minichromosome maintenance complex component 3 associated protein; Minichromosome maintenance deficient 3 associated protein; Minichromosome maintenance protein 3 associated protein; Protein GANP; SAC3;

免疫原

免疫原:
Uniprot:

O60318(GANP_HUMAN) >>Visit HPA database.

遺伝子(ID):
MCM3AP(8888)
発現特異性:
O60318 GANP_HUMAN:

Widely expressed (PubMed:11024281). Up-regulated in germinal center B-cells in tonsils (at protein level) (PubMed:11024281).

タンパク質の説明:
GANP is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3 region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF- B, and p53 consensus transcription factor binding sites are located.
タンパク質配列:
MNPTNPFSGQQPSAFSASSSNVGTLPSKPPFRFGQPSLFGQNSTLSGKSSGFSQVSSFPASSGVSHSSSVQTLGFTQTSSVGPFSGLEHTSTFVATSGPSSSSVLGNTGFSFKSPTSVGAFPSTSAFGQEAGEIVNSGFGKTEFSFKPLENAVFKPILGAESEPEKTQSQIASGFFTFSHPISSAPGGLAPFSFPQVTSSSATTSNFTFSKPVSSNNSLSAFTPALSNQNVEEEKRGPKSIFGSSNNSFSSFPVSSAVLGEPFQASKAGVRQGCEEAVSQVEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNKEVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRSESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKKGKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGASSLLNKSSPVKKPSLLKAHQFEGDSFDSASEGSEGLGPCVLSLSTLIGTVAETSKEKYRLLDQRDRIMRQARVKRTDLDKARTFVGTCLDMCPEKERYMRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPLPVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLSLNKGDILREVQQFHPAVRNSSEVKFAVQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLFRDCEEATDFLTCHGLTVSDGCVELNRSAFLEPEGLSKTRKSVFITRKLTVSVGEIVNGGPLPPVPRHTPVCSFNSQNKYIGESLAAELPVSTQRPGSDTVGGGRGEECGVEPDAPLSSLPQSLPAPAPSPVPLPPVLALTPSVAPSLFQLSVQPEPPPPEPVPMYSDEDLAQVVDELIQEALQRDCEEVGSAGAAYAAAALGVSNAAMEDLLTAATTGILRHIAAEEVSKERERREQERQRAEEERLKQERELVLSELSQGLAVELMERVMMEFVRETCSQELKNAVETDQRVRVARCCEDVCAHLVDLFLVEEIFQTAKETLQELQCFCKYLQRWREAVTARKKLRRQMRAFPAAPCCVDVSDRLRALAPSAECPIAEENLARGLLDLGHAGRLGISCTRLRRLRNKTAHQMKVQHFYQQLLSDVAWASLDLPSLVAEHLPGRQEHVFWKLVLVLPDVEEQSPESCGRILANWLKVKFMGDEGSVDDTSSDAGGIQTLSLFNSLSSKGDQMISVNVCIKVAHGALSDGAIDAVETQKDLLGASGLMLLLPPKMKSEDMAEEDVYWLSALLQLKQLLQAKPFQPALPLVVLVPSPGGDAVEKEVEDGLMLQDLVSAKLISDYTVTEIPDTINDLQGSTKVLQAVQWLVSHCPHSLDLCCQTLIQYVEDGIGHEFSGRFFHDRRERRLGGLASQEPGAIIELFNSVLQFLASVVSSEQLCDLSWPVTEFAEAGGSRLLPHLHWNAPEHLAWLKQAVLGFQLPQMDLPPLGAPWLPVCSMVVQYASQIPSSRQTQPVLQSQVENLLHRTYCRWKSKSPSPVHGAGPSVMEIPWDDLIALCINHKLRDWTPPRLPVTSEALSEDGQICVYFFKNDLKKYDVPLSWEQARLQTQKELQLREGRLAIKPFHPSANNFPIPLLHMHRNWKRSTECAQEGRIPSTEDLMRGASAEELLAQCLSSSLLLEKEENKRFEDQLQQWLSEDSGAFTDLTSLPLYLPQTLVSLSHTIEPVMKTSVTTSPQSDMMREQLQLSEATGTCLGERLKHLERLIRSSREEEVASELHLSALLDMVDI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Bovine
90
Sheep
90
Pig
80
Dog
80
Rabbit
80
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60318 基板として

Site PTM Type Enzyme
S46 Phosphorylation
K147 Ubiquitination
S300 Phosphorylation
T336 Phosphorylation
K347 Methylation
S408 Phosphorylation
T412 Phosphorylation
S417 Phosphorylation
S422 Phosphorylation
S425 Phosphorylation
S430 Phosphorylation
K440 Ubiquitination
R449 Methylation
K458 Ubiquitination
S508 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
T530 Phosphorylation
S538 Phosphorylation
S557 Phosphorylation
S574 Phosphorylation
K644 Ubiquitination
K674 Ubiquitination
K781 Ubiquitination
K787 Acetylation
K787 Ubiquitination
Y864 Phosphorylation
Y873 Phosphorylation
K947 Ubiquitination
T955 Phosphorylation
S1101 Phosphorylation
Y1106 Phosphorylation
S1139 Phosphorylation
K1140 Ubiquitination
K1194 Ubiquitination
K1241 Acetylation
K1386 Ubiquitination
K1448 Ubiquitination
K1512 Ubiquitination
K1527 Ubiquitination
K1785 Ubiquitination
K1801 Ubiquitination
K1813 Acetylation
S1836 Phosphorylation
S1847 Phosphorylation
T1848 Phosphorylation
K1873 Ubiquitination
S1922 Phosphorylation
T1924 Phosphorylation
T1925 Phosphorylation
S1926 Phosphorylation

研究背景

機能:

As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.

Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.

細胞の位置付け:

Nucleus envelope. Nucleus>Nuclear pore complex. Nucleus>Nucleoplasm. Chromosome.
Note: Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:23591820, PubMed:22307388). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018).

Cytoplasm. Nucleus.
Note: Translocates into the nucleus in the presence of MCM3 (PubMed:12226073). Associates with chromatin possibly through interaction with MCM3 (PubMed:12226073).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Up-regulated in germinal center B-cells in tonsils (at protein level).

サブユニット構造:

Isoform GANP: Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, 2 copies of ENY2, PCID2, SEM1/DSS1, and either centrin CETN2 or centrin CETN3. The TREX-2 complex also associates with ALYREF/ALY. Interacts with RNA polymerase II subunit POLR2A and with the transcription elongation factor SUPT5H/SPT5. Interacts (via FG-repeats) with NXF1; this interaction is not mediated by RNA. Isoform GANP interacts with nuclear envelope proteins NUP62, NUP153 and RANBP2/NUP358; interaction with NUP153 is required for full localization at the nuclear pore complex. Interacts with several RNA helicases, including DHX9, DDX21, and DDX39A/DDX39, and with DNA topoisomerase TOP2A. Directly interacts with AICDA/AID. Interacts with the glucocorticoid receptor NR3C1. Isoform MCM3AP: Interacts with the glucocorticoid receptor NR3C1. Interacts with MCM3; this interaction leads to MCM3 acetylation.

タンパク質ファミリー:

Belongs to the SAC3 family.

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